Surface Plasmon Resonance (SPR) imaging is a label free method that can be used for the monitoring of the hybridization kinetics between oligonucleotides probes immobilized onto the Au surface and unlabelled complementary targets. In this work an automated SPR imaging-based framework on a PC architecture has been used as an optical biosensor, allowing high reliability real-time analysis of DNA-DNA interactions occurring at the biochip surface.