Electrochemical non-enzymatic detections of glucose and fructose were based on gold nanoparticles (AuNPs) onto graphene paper. Electrodes based on AuNPs have been obtained inducing dewetting, by thermal (furnace) or by laser, of sputter deposited 8 nm-thick Au layer onto graphene paper. The electrodes were characterized by Scanning Electron Microscopy, Micro Raman Spectroscopy, X-ray Diffraction, Rutherford back-scattering Spectroscopy and Cyclic Voltammetry. The main difference exhibited by thermal and laser dewetting processes lies in the size and shape of the resulting gold nanoparticles. Laser dewetting originates smaller particles than that obtained by thermal dewetting. The particles are almost spherical and mainly localized onto graphene nanoplatelets. The size of AuNPs is in the ranges 10–150 nm. Electrodes obtained by thermal process present gold nanostructures characterized by …