A design of a µDMFC using a polymeric substrate was reported. Different cell configurations were realized, tested and evaluated. It was observed that the open circuit voltage varied significantly as a function of the membrane humidification and distance between anode and cathode channels in this planar design. In the presence of a large distance between the anode and cathode channel, the OCV reached 0.97 V. This high OCV reflects the absence of methanol cross-over due to the specific planar design. Unfortunately, the cell resistance limited the performance. A maximum power density (IR-free) of 1.1 mW cm-2 (active area) was achieved at room temperature with the smallest distance between anode and cathode (0.25 mm).