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Type: 
Conference
Description: 
In this study label-free a SPR immunosensor and a protein array based methods were used for their application in bacterial detection. L. monocytogenes, one of the most difficult to treat bacteria, was used as model pathogen. While the use of DNA arrays for bacteria detection and identification is largely documented, no studies are available on protein array (PA) based bacteria detection. In protein array approach an affinity-purified monoclonal antibody was used as capture antibody. Protein array detection limit was of 102-103CFU/mL. SPR immunoassays were prepared by chemically binding L. monocytogenes cells on a proper gold substrate. After immobilization of antigen on gold substrate, a further incubation with anti-L. monocytogenes antibodies was performed. The technique was revealed as powerful and fast method for the monitoring of binding between the investigated antigen and antibodies. On the other …
Publisher: 
IEEE
Publication date: 
26 Oct 2008
Authors: 

Oscar Fernando D'Urso, Marialuce Daniela De Blasi, Maria Grazia Manera, Maria Francesca Latronico, Roberto Rella, Palmiro Poltronieri

Biblio References: 
Pages: 458-461
Origin: 
SENSORS, 2008 IEEE