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Field flow fractionation dielectrophoretic (FFF-DEP) devices are currently used, among the others, for the separation of tumor cells from healthy blood cells. To this end specific suspension/elution buffers (EBs), with reduced conductivity (with respect to that of the cell cytoplasm) are generally used. In this paper we investigate the long-term alterations of the cells and elution buffers. We find that the EB conductivity is critically modified within few minutes after cells suspension. In turn, this modification results in a change the ideal separation frequency of the FFF-DEP device. On the other hand we prove that DEP manipulation is preserved for more than three hours for cells suspended in the considered EBs.
Publication date: 
1 Mar 2016

Rossana Di Martino, Massimo Camarda, Michele Cascio, Michele Gallo, Antonino Magliano, Salvatore Baldo, Alessandra Romano, Luigi Minafra, Giusi I Forte, Giorgio Russo, Maria Carla Gilardi, Francesco Di Raimondo, Silvia Scalese, Antonino La Magna

Biblio References: 
Volume: 7 Pages: 162-167
Sensing and bio-sensing research