A complete label-free and non-destructive analysis of semen quality (in terms of morphology and biochemical structure integrity) is required before artificial insemination. An optical approach based on digital holography and Raman spectroscopy was proposed and simultaneously applied for the fast characterization of single selected sperm cells. In this work, we have used this approach to simultaneously study biochemical and morphological characteristics of human sperm cells irradiated with different green laser powers. Severe ...
6 Jun 2016
Photonic Technologies (Fotonica 2016), 18th Italian National Conference on