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In the last years, nanopore technology has been increasingly exploited for biomolecule detection and analysis. Recently, the main focus of the research has moved from the study of nucleic acids to the analysis of proteins and DNA-protein complexes. In this paper, chemically functionalized solid-state nanopore has been used to recognize Nuclear Factor-kappa B proteins (NF-κB), that are involved in several disorders and inflammation processes, so that their identification is of crucial importance for prognostic applications. In particular, we show that it is possible to electrically detect the specific interaction between p50, a protein belonging to the NF-κB family, and dsLNA probe molecules covalently attached to the surface of a FIB fabricated SiN pore. The obtained results have been compared with those related to BSA protein, which does not interact with the used probes. Finally, the potential of the device has been …
Publication date: 
15 Feb 2015

Paola Fanzio, Valentina Mussi, Michele Menotta, Giuseppe Firpo, Luca Repetto, Patrizia Guida, Elena Angeli, Mauro Magnani, Ugo Valbusa

Biblio References: 
Volume: 64 Pages: 219-226
Biosensors and Bioelectronics