Multianalyte biosensor patch based on polymeric microneedles (MNs) have been fabricated by photolithography of poly (ethylene glycol) diacrylate (PEGDA) and have been dynamically characterized by means of quartz crystal microbalance and electrochemical measurements. The resulting flexible device acts as multianalyte working electrodes detecting glucose and lactic acid into interstitial liquid by means of redox reaction with glucose oxidase (GOx) and lactose oxidase (LOx) enzymes. Sensitivities of the order of nA mM− 1 in the mM range are revealed in both case, after five minutes of swelling time and ten second of interaction.