-A A +A
A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH presents new opportunities for studying morphological aspect of cells and tissues non-invasively, quantitatively and without the need for staining or tagging, while RS is a very specific technique allowing the biochemical analysis of cellular components with a spatial resolution in the sub-micrometer range. In this paper, morphological and biochemical bovine sperm cell alterations were studied using these techniques. In addition, a complementary DH and RS study was performed to identify X-and Y-chromosome-bearing sperm cells. We demonstrate that the two techniques together are a powerful and highly efficient tool elucidating some important criterions for sperm morphological selection and sex-identification, overcoming many of the limitations associated with existing protocols. View Full-Text
Multidisciplinary Digital Publishing Institute
Publication date: 
1 Jun 2015

Maria Antonietta Ferrara, Giuseppe Di Caprio, Stefano Managò, Annalisa De Angelis, Luigi Sirleto, Giuseppe Coppola, Anna Chiara De Luca

Biblio References: 
Volume: 5 Issue: 2 Pages: 141-157