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X-and Y-chromosome-bearing sperm cell sorting is of great interest, especially for animal production management systems and genetic improvement programs. Here, we demonstrate an optical method based on Raman spectroscopy to separate X-and Y-chromosome-bearing sperm cells, overcoming many of the limitations associated with current sex-sorting protocols. A priori Raman imaging of bull spermatozoa was utilized to select the sampling points (head-neck region), which were then used to discriminate cells based on a spectral classification model. Main variations of Raman peaks associated with the DNA content were observed together with a variation due to the sex membrane proteins. Next, we used principal component analysis to determine the efficiency of our device as a cell sorting method. The results (> 90% accuracy) demonstrated that Raman spectroscopy is a powerful candidate for the …
IOP Publishing
Publication date: 
9 Apr 2014

AC De Luca, S Managó, MA Ferrara, I Rendina, L Sirleto, R Puglisi, D Balduzzi, A Galli, P Ferraro, G Coppola

Biblio References: 
Volume: 11 Issue: 5 Pages: 055604
Laser Physics Letters