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In this work, we present successfully realization of a nonlinear microscope, not purchasable in commerce, based on stimulated Raman scattering. It is obtained by the integration of a femtosecond SRS spectroscopic setup with an inverted research microscope equipped with a scanning unit. Taking account of strength of vibrational contrast of SRS, it provides label-free imaging of single cell analysis. Validation tests on images of polystyrene beads are reported to demonstrate the feasibility of the approach. In order to test the microscope on biological structures, we report and discuss the label-free images of lipid droplets inside fixed adipocyte cells.
International Society for Optics and Photonics
Publication date: 
16 May 2017

Annalisa D'Arco, Maria Antonietta Ferrara, Maurizio Indolfi, Vitaliano Tufano, Luigi Sirleto

Biblio References: 
Volume: 10228 Pages: 102280S
Nonlinear Optics and Applications X