The small intestine is the central component of the gastrointestinal (GI) tract (gut) where nutrients are absorbed into the body. Its functional structure is mainly based on its extremely extended surface area, further increased by a specific carpet of villi, responsible for the translocation of nutrients from the GI lumen into the bloodstream. Also, in the small intestine, the absorption processes of the orally administered drugs are basically related to the pharmacokinetics [1]. The deficit of cell culture methods to maintain in vivo–like functions forces researchers to optimize and apply methods in which cells are seeded and cultured under controlled and dynamic fluid flow [2]. Moreover, the lack of predictive human organ models has increased the necessity of approaches for proper mimicking of organ function in vitro, studying physiological parameters that regard mechanical, chemical and physical stimuli crucial for …