Aim: Most of the research efforts in magnetic targeting have been focused on the development of magnetic nanovectors, while the investigation of methods for tracking their magnetic targeting efficiency remains inappropriately addressed. We propose herein a miniaturized approach for appraising magnetophoretic mobility at the nanoscale. Materials & methods: A simple and easy-to-use chamber including a microtip as a magnetic attractor was developed to perform magnetophoretic measurement at the size scale of nano-objects, and under bright field or fluorescence microscopy. Different sets of magnetic nanocontainers were produced and their magnetophoretic mobility was investigated. Real-time observations of the Brownian motion of the nanocontainers were also carried out for simultaneous size determination. Results: Attraction of the nanocontainers at the microtip is demonstrated as a qualitative method …