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Type: 
Book
Description: 
Electrochemical on-chip deposition of proteins for further biodetection of several analytes by multiplexed chronoamperometry in a continuous flow µ-chamber is presented. Recently, an original deposition procedure was developed obtaining oriented and reversible immobilisation of an engineered photosystem II with an histidine-tag and its on-chip purification from bacterial crude extracts. The electrochemically assisted method started with the deposition of cysteamin on gold or platinum surfaces and further chemical synthesis of a spacer with a Ni-NTA end capable to link the engineered proteins. This technique is applied on a µ-chip assembled in a µ-chamber with an histidine-tagged alkaline phosphatase. Electrochemical deposition allowed this protein to be addressed only on a specific electrode in the µ-array in few minutes and under flow condition (flow-rate=10 µl/min).
Publisher: 
Publication date: 
1 Jan 2005
Authors: 

J MALY, M ILIE, V FOGLIETTI, E CIANCI, A MINOTTI, B LANZA, A MASCI, W VASTARELLA, R PILLOTON

Biblio References: 
Pages: 8-13
Origin: 
Sensors And Microsystems