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This paper summarizes the achievements in the 3D reconstruction of microscopic specimens through the tomographic algorithm applied to a set of projection\images obtained in the SEM. This approach is complementary to the serial‐sectioning and the slice‐and‐view methods presently implemented in the SEM platform, and benefits from a compressed sensing approach to refine the reconstruction from a limited number of projections.A Si‐based electron detector has been specifically developed for the purpose of operating the microscope in the scanning‐transmission imaging mode for the tomographic application, and the detection strategy has been tailored in order to maintain the projection requirement over the large tilt range, a requirement needed for the reconstruction workflow [1]. Either inorganic or biological samples have been investigated to demonstrate the adaptability of the compressed sensing …
Wiley‐VCH Verlag GmbH & Co. KGaA
Publication date: 
30 Nov 2016

Ferroni Matteo, Alberto Signoroni, Andrea Sanzogni, Andrea Migliori, Luca Ortolani, Vittorio Morandi

Biblio References: 
Pages: 47-48
European Microscopy Congress 2016: Proceedings