Astrocytes, essential components of the central nervous system (CNS), perform diverse functions such as providing structural support, maintaining ion homeostasis, forming a glial scar after injury, contributing to the blood-brain barrier, and providing neuroprotection, tasks that are critical to proper CNS function. Astrocytes comprise of the soma with radially arranged extendable processes. They display distinctive morphological characteristics to perform their specific functions successfully. Abnormalities in astrocyte morphology have been linked to various neurological disorders. Despite their acknowledged significance, our comprehension of astrocytes remains incomplete, particularly regarding their intricate morphology. In the past, astrocytes were visualized using fluorescence microscopy. Using dye has several disadvantages, including increased chances of photobleaching, perturbations to the system, and not …