In microscopy high magnifications are achievable for investigating micro-objects but the paradigm is that higher is the required magnification lower the depth of focus is. For an object having a 3D complex shape only a portion of it appears in well focus to the observer that essentially is looking at a single image plane. If an accurate analysis of the whole object has to be performed it is necessary to have a single sharp image in which all details of the object. We propose an holographic microscope to construct an EFI without any …