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Type: 
Journal
Description: 
Coupling digital holography with high-specific Raman spectroscopy is an attractive means of identifying biochemical changes in cells by both physical topography and spectroscopy. We have used this approach to simultaneously study biochemical and morphological characteristics of human sperm cells irradiated with green laser radiation. Severe spermatozoa variations associated with a topological redistribution of the sample and a gradual decrease in the Raman signal intensity were detected in a label-free configuration. Importantly, at laser fluences where no morphological alterations were detected (30 MJ/cm 2 ), high specific spectral variations were monitored to evaluate the cell photodegradation.
Publisher: 
IEEE
Publication date: 
30 Oct 2015
Authors: 

Maria Antonietta Ferrara, Annalisa De Angelis, Anna Chiara De Luca, Gianfranco Coppola, Brian Dale, Giuseppe Coppola

Biblio References: 
Volume: 22 Issue: 3 Pages: 27-34
Origin: 
IEEE Journal of Selected Topics in Quantum Electronics